The development of a hiPSC-based platform to identify tissue-dependencies of IDH1 R132H
N. Z. Mehjardi, J. Kessler, A. Y. Sanin, D. Picard, P. Westhoff, Ann-Christin Nickel, C. Uhlmann, W. Shi, H. J. Steiger, M. Remke, I. Fischer, D. Vordermark, R. S. Croner, U. D. Kahlert · 2023
Read The development of a hiPSC-based platform to identify tissue-dependencies of IDH1 R132H by N. Z. Mehjardi, J. Kessler, A. Y. Sanin, D. Picard, P. Westhoff, Ann-Christin Nickel, C. Uhlmann, W. Shi, H. J. Steiger, M. Remke, I. Fischer, D. Vordermark, R. S. Croner, U. D. Kahlert (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10716401.txt.
Description
Mehjardi and co-authors build a stem cell platform to find tissue dependencies of a cancer gene fault. Their 2023 paper deals with a mutation in a gene called IDH1, at a hot spot known as R132H. Such faults occur in many tumors. Patient derived tumor cultures are the classic tool for drug work. Sorting true cancer stem cells from them, though, is hard and contested.
The team transformed single clone human stem cells with the IDH1 fault and a second fault in a guard gene. They used glial tumors as the model disease. Drug tests on the engineered stem cells found kinase drugs that preferentially hit cells with the fault. Brain stem cells with the same fault, by contrast, grew more resistant. That contrast shows a tissue dependent effect.
Catalog record
| CREATOR | N. Z. Mehjardi, J. Kessler, A. Y. Sanin, D. Picard, P. Westhoff, Ann-Christin Nickel, C. Uhlmann, W. Shi, H. J. Steiger, M. Remke, I. Fischer, D. Vordermark, R. S. Croner, U. D. Kahlert |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 37 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |