An integrated workflow for quantitative analysis of the newly synthesized proteome
Toman Borteçen, Torsten Müller, Jeroen Krijgsveld · 2023
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Description
Toman Borteçen, Torsten Müller, and Jeroen Krijgsveld streamline the hunt for proteins a cell has just made, in a methods paper published in two thousand twenty-three. Newly synthesized proteins reveal a cell's immediate response to a stimulus. Labeling them with clickable, isotope-coded amino acids works, but protocols were too convoluted for routine use.
Their workflow optimizes preparation, mass spectrometry, and analysis into a semi-automated pipeline. It needs less input material and captures triple-labeled samples with high accuracy and throughput. Applying it to interferon stimulation showed rapid induction of response proteins over time. Methods papers multiply a whole field's experiments; a usable nascent-proteome pipeline turns a specialist trick into a standard measurement.
Catalog record
| CREATOR | Toman Borteçen, Torsten Müller, Jeroen Krijgsveld |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 88 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |