Purification of Human Cytoplasmic Actins From Saccharomyces cerevisiae
Brian K Haarer, David C Amberg, Jessica L Henty-Ridilla · 2023
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Description
Brian Haarer, David Amberg, and Jessica Henty-Ridilla present a 2023 protocol for making pure human actin in baker's yeast. Actin is the workhorse protein of cell structure and motion, and the non-muscle forms, beta and gamma, are hard to get pure: most studies still use muscle actin. The team bred yeast strains whose only actin is one untagged human form at a time.
From those strains, standard lab steps yield single, clean isoforms with no tags to strip off, a flaw in earlier systems. The authors stress the ease of growing yeast and the fit with long-standing purification recipes. Biochemists gain a steady source for testing how each isoform and its partners behave.
Catalog record
| CREATOR | Brian K Haarer, David C Amberg, Jessica L Henty-Ridilla |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 59 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |