Enhancing the Conventional Culture: the Evaluation of Several Culture Media and Growth Conditions Improves the Isolation of Ruminal Bacteria
Lina Marcela Botero Rute, Alejandro Caro-Quintero, Alejandro Acosta-González · 2023
Read Enhancing the Conventional Culture: the Evaluation of Several Culture Media and Growth Conditions Improves the Isolation of Ruminal Bacteria by Lina Marcela Botero Rute, Alejandro Caro-Quintero, Alejandro Acosta-González (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10713758.txt.
Description
Lina Botero Rute and co-authors tune the recipe book for growing the bacteria of a cow's rumen in a dish. The rumen's microbes digest what the cow cannot, but most refuse to grow in the laboratory, hiding their ecology and their uses. Their 2023 paper tests eight culture media, three dilutions, and two incubation times against one another, checking who grew by DNA sequencing.
No single medium recovered everything, and the cultures skewed the community badly: the two dominant groups of the real rumen swapped their shares almost exactly on the plates. Dilution moved the grown community strongly; incubation time did not. Two media, called CAN and KNT, came closest to the rumen's own composition and were selected for the work ahead. The authors hand microbiologists a tested shortlist, and a warning that the dish always edits the community it claims to copy.
Catalog record
| CREATOR | Lina Marcela Botero Rute, Alejandro Caro-Quintero, Alejandro Acosta-González |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 46 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |