Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging
Ueh-Ting Tim Wang, Xuejiao Tian, Yae-Huei Liou, Sue-Ping Lee, Hsiao-Tang Hu, Chieh-Han Lu, Po-Ting Lin, Ya-Jen Cheng, Peilin Chen, Bi-Chang Chen · 2023
Read Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging by Ueh-Ting Tim Wang, Xuejiao Tian, Yae-Huei Liou, Sue-Ping Lee, Hsiao-Tang Hu, Chieh-Han Lu, Po-Ting Lin, Ya-Jen Cheng, Peilin Chen, Bi-Chang Chen (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10713663.txt.
Description
Ueh-Ting Wang and co-authors improve, in a 2023 methods paper, a microscopy trick that swells cells in a gel so fine detail becomes visible under an ordinary microscope. The method, expansion microscopy, gives super-clear views, but the swelling and digestion steps fade the glow of labeled structures, above all in thick three-dimensional samples.
Their version swaps in a gentler enzyme, trypsin, to spare proteins, and adds a signal-boosting step that brightens labels. The result kept more protein signal for cell structures such as the endoplasmic reticulum and mitochondria. It also revealed full three-dimensional membrane webs across whole swollen cells. Simpler steps may help the method spread to more labs.
Catalog record
| CREATOR | Ueh-Ting Tim Wang, Xuejiao Tian, Yae-Huei Liou, Sue-Ping Lee, Hsiao-Tang Hu, Chieh-Han Lu, Po-Ting Lin, Ya-Jen Cheng, Peilin Chen, Bi-Chang Chen |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |