Generating high-quality plant and fish reference genomes from field-collected specimens by optimizing preservation
Jeremiah J. Minich, Malia L. Moore, Nicholas A. Allsing, Anthony Aylward, Emily R. Murray, Loi Tran, Todd P. Michael · 2023
Read Generating high-quality plant and fish reference genomes from field-collected specimens by optimizing preservation by Jeremiah J. Minich, Malia L. Moore, Nicholas A. Allsing, Anthony Aylward, Emily R. Murray, Loi Tran, Todd P. Michael (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10710401.txt.
Description
Jeremiah Minich leads a team on saving field samples for genome work. Their study from twenty twenty-three deals with preservation methods. Poor storage degrades DNA and blocks good genomes.
Storage fluids, temperatures, and times were compared head to head. Fish and plant species from the field were used. Ethanol guarded fish blood well for weeks at room heat. Plant tissue kept well cold for a few weeks. Genomes built from the oldest samples met project standards. Methylation rates were checked against frozen controls. Remote sampling may no longer need deep freezing.
Catalog record
| CREATOR | Jeremiah J. Minich, Malia L. Moore, Nicholas A. Allsing, Anthony Aylward, Emily R. Murray, Loi Tran, Todd P. Michael |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |