Comparative RNA-Seq Analysis Revealed Tissue-Specific Splicing Variations during the Generation of the PDX Model
Eun Ji Lee, Seung-Jae Noh, Huiseon Choi, Min Woo Kim, Su Jin Kim, Yeon Ah Seo, Ji Eun Jeong, Inkyung Shin, Jong-Seok Kim, Jong-Kwon Choi, Dae-Yeon Cho, Suhwan Chang · 2023
Read Comparative RNA-Seq Analysis Revealed Tissue-Specific Splicing Variations during the Generation of the PDX Model by Eun Ji Lee, Seung-Jae Noh, Huiseon Choi, Min Woo Kim, Su Jin Kim, Yeon Ah Seo, Ji Eun Jeong, Inkyung Shin, Jong-Seok Kim, Jong-Kwon Choi, Dae-Yeon Cho, Suhwan Chang (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10707456.txt.
Description
Growing a patient's tumor in a mouse is a common way to test cancer ideas, but the graft may drift from the original. Eun Ji Lee and co-authors compare gene activity in four tumor-and-graft pairs using RNA sequencing. They look at which genes are on, and how gene messages are spliced together.
The broad mix of RNA types stayed similar between each tumor and its graft. Splicing, though, changed clearly in the grafts, mostly through skipped segments of genes. Lab spot-checks confirmed the shift. Tissue identity markers mostly tracked together, except in stomach cancer, where the graft ran opposite to the source. The authors warn that splicing drift is a hidden variable in mouse graft studies. Results from such models should be read with that drift in mind.
Catalog record
| CREATOR | Eun Ji Lee, Seung-Jae Noh, Huiseon Choi, Min Woo Kim, Su Jin Kim, Yeon Ah Seo, Ji Eun Jeong, Inkyung Shin, Jong-Seok Kim, Jong-Kwon Choi, Dae-Yeon Cho, Suhwan Chang |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 21 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |