Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis
Aurora Diotallevi, Gloria Buffi, Simone Barocci, Marcello Ceccarelli, Daniela Bencardino, Francesca Andreoni, Chiara Orlandi, Marilisa Ferri, Daniela Vandini, Stefano Menzo, Eugenio Carlotti, Anna Casabianca, Mauro Magnani, Luca Galluzzi · 2023
Read Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis by Aurora Diotallevi, Gloria Buffi, Simone Barocci, Marcello Ceccarelli, Daniela Bencardino, Francesca Andreoni, Chiara Orlandi, Marilisa Ferri, Daniela Vandini, Stefano Menzo, Eugenio Carlotti, Anna Casabianca, Mauro Magnani, Luca Galluzzi (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10703772.txt.
Description
Aurora Diotallevi and co-authors build a fast test that names coronavirus variants without full genome reads. New variants of the virus kept driving waves of illness through the pandemic years. Full genome reading is slow, costly, and needs rare gear and staff. Their test uses PCR plus a melt curve step that reads how gene pieces melt apart.
Six assays on the spike gene were run on 120 swab samples. The tests matched standard PCR calls with full accuracy on virus presence. They named the variant right in 104 of 106 positive samples, from the first strain to later Omicron lines. Ten samples took about three hours at under ten euros each. The team offers the method for local watch programs.
Catalog record
| CREATOR | Aurora Diotallevi, Gloria Buffi, Simone Barocci, Marcello Ceccarelli, Daniela Bencardino, Francesca Andreoni, Chiara Orlandi, Marilisa Ferri, Daniela Vandini, Stefano Menzo, Eugenio Carlotti, Anna Casabianca, Mauro Magnani, Luca Galluzzi |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |