Optogenetic Microwell Array Screening System: A High-Throughput Engineering Platform for Genetically Encoded Fluorescent Indicators
Michael Rappleye, Sarah J Wait, Justin Daho Lee, Jamison C Siebart, Lily Torp, Netta Smith, Jeanot Muster, Kenneth A Matreyek, Douglas M Fowler, Andre Berndt · 2023
Read Optogenetic Microwell Array Screening System: A High-Throughput Engineering Platform for Genetically Encoded Fluorescent Indicators by Michael Rappleye, Sarah J Wait, Justin Daho Lee, Jamison C Siebart, Lily Torp, Netta Smith, Jeanot Muster, Kenneth A Matreyek, Douglas M Fowler, Andre Berndt (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10683761.txt.
Description
A peer-reviewed research article from Europe PMC (2023). Genetically encoded fluorescent indicators (GEFIs) are protein-based optogenetic tools that change their fluorescence intensity when binding specific ligands in cells and tissues. GEFI encoding DNA can be expressed in cell subtypes while monitoring cellular physiological responses. However, engineering GEFIs with physiological sensitivity and pharmacological specificity often requires iterative optimization through trial-and-error mutagenesis while assessing their biophysical function in vitro one by one.
Catalog record
| CREATOR | Michael Rappleye, Sarah J Wait, Justin Daho Lee, Jamison C Siebart, Lily Torp, Netta Smith, Jeanot Muster, Kenneth A Matreyek, Douglas M Fowler, Andre Berndt |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 60 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |