Up Front Unfolded Protein Response Combined with Early Protein Secretion Pathway Engineering in Yarrowia lipolytica to Attenuate ER Stress Caused by Enzyme Overproduction
Xingyu Zhu, Moying Li, Rui Zhu, Yu Xin, Zitao Guo, Zhenghua Gu, Liang Zhang, Zhongpeng Guo · 2023
Read Up Front Unfolded Protein Response Combined with Early Protein Secretion Pathway Engineering in Yarrowia lipolytica to Attenuate ER Stress Caused by Enzyme Overproduction by Xingyu Zhu, Moying Li, Rui Zhu, Yu Xin, Zitao Guo, Zhenghua Gu, Liang Zhang, Zhongpeng Guo (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10670989.txt.
Description
Xingyu Zhu and co-authors (2023) engineered the yeast Yarrowia lipolytica to secrete proteins with less cell stress. Factories that push this yeast to overproduce enzymes fill its folding compartment with misshapen proteins. The resulting stress slows growth and cuts output. The team tested helpers from two stages of the secretion path: the machinery that threads new proteins into the compartment, and the chaperones that fold them. They tracked two products, the yeast's own lipase and a bacterial enzyme.
Strengthening the backup threading path raised output of both proteins. One folding helper, the exchange factor Sls1p, helped both products as well. Combined with stress-response switches, the fixes doubled lipase secretion in one strain. Another mix raised the bacterial enzyme's output by ninety percent. The engineered cells grew almost as fast as wild yeast. The authors present the recipe as a step toward hardier protein factories.
Catalog record
| CREATOR | Xingyu Zhu, Moying Li, Rui Zhu, Yu Xin, Zitao Guo, Zhenghua Gu, Liang Zhang, Zhongpeng Guo |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 31 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |