Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study
Thailin Lao, Omar Farnos, Alexi Bueno, Anays Alvarez, Elsa Rodríguez, Julio Palacios, Kathya Rashida de la Luz, Amine Kamen, Yamila Carpio, Mario Pablo Estrada, Francesco Bianco, Joanna Szczepanek · 2023
Read Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study by Thailin Lao, Omar Farnos, Alexi Bueno, Anays Alvarez, Elsa Rodríguez, Julio Palacios, Kathya Rashida de la Luz, Amine Kamen, Yamila Carpio, Mario Pablo Estrada, Francesco Bianco, Joanna Szczepanek (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10669214.txt.
Description
In a previous work, we proposed a vaccine chimeric antigen based on the fusion of the SARS-CoV-2 N protein to the extracellular domain of the human CD40 ligand (CD154). This vaccine antigen was named N-CD protein and its expression was carried out in HEK-293 stably transfected cells, grown in adherent conditions and serum-supplemented medium.
Catalog record
| CREATOR | Thailin Lao, Omar Farnos, Alexi Bueno, Anays Alvarez, Elsa Rodríguez, Julio Palacios, Kathya Rashida de la Luz, Amine Kamen, Yamila Carpio, Mario Pablo Estrada, Francesco Bianco, Joanna Szczepanek |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 59 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |