Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons
Saadia Hasan, Michael S Fernandopulle, Stewart W Humble, Ashley M Frankenfield, Haorong Li, Ryan Prestil, Kory R Johnson, Brent J Ryan, Richard Wade-Martins, Michael E Ward, Ling Hao · 2023
Read Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons by Saadia Hasan, Michael S Fernandopulle, Stewart W Humble, Ashley M Frankenfield, Haorong Li, Ryan Prestil, Kory R Johnson, Brent J Ryan, Richard Wade-Martins, Michael E Ward, Ling Hao (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10655356.txt.
Description
Saadia Hasan and co-authors map what goes wrong inside neurons that lack progranulin, a protein tied to dementia. More than seventy gene mutations cut this protein down, and the cell's recycling centers, the lysosomes, suffer for it. The team built a multi-tool protein survey to watch those centers closely.
They tagged and fished out whole lysosomes from human lab-grown neurons and mouse brains. A dynamic labeling method clocked how long each protein lives, a first for these neurons. Live imaging joined the protein maps. The combined view shows how progranulin loss reshapes lysosome contents, partners, and protein turnover.
Catalog record
| CREATOR | Saadia Hasan, Michael S Fernandopulle, Stewart W Humble, Ashley M Frankenfield, Haorong Li, Ryan Prestil, Kory R Johnson, Brent J Ryan, Richard Wade-Martins, Michael E Ward, Ling Hao |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 67 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |