CHEX-seq detects single-cell genomic single-stranded DNA with catalytical potential
Youtao Lu, Jaehee Lee, Jifen Li, Srinivasa Rao Allu, Jinhui Wang, HyunBum Kim, Kevin L Bullaughey, Stephen A Fisher, C Erik Nordgren, Jean G Rosario, Stewart A Anderson, Alexandra V Ulyanova, Steven Brem, H Isaac Chen, John A Wolf, M Sean Grady, Sergei A Vinogradov, Junhyong Kim, James Eberwine · 2023
Read CHEX-seq detects single-cell genomic single-stranded DNA with catalytical potential by Youtao Lu, Jaehee Lee, Jifen Li, Srinivasa Rao Allu, Jinhui Wang, HyunBum Kim, Kevin L Bullaughey, Stephen A Fisher, C Erik Nordgren, Jean G Rosario, Stewart A Anderson, Alexandra V Ulyanova, Steven Brem, H Isaac Chen, John A Wolf, M Sean Grady, Sergei A Vinogradov, Junhyong Kim, James Eberwine (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10645931.txt.
Description
A peer-reviewed research article from Europe PMC (2023). Genomic DNA (gDNA) undergoes structural interconversion between single- and double-stranded states during transcription, DNA repair and replication, which is critical for cellular homeostasis. We describe “CHEX-seq” which identifies the single-stranded DNA (ssDNA) in situ in individual cells. CHEX-seq uses 3’-terminal blocked, light-activatable probes to prime the copying of ssDNA into complementary DNA that is sequenced, thereby reporting the genome-wide single-stranded chromatin landscape.
Catalog record
| CREATOR | Youtao Lu, Jaehee Lee, Jifen Li, Srinivasa Rao Allu, Jinhui Wang, HyunBum Kim, Kevin L Bullaughey, Stephen A Fisher, C Erik Nordgren, Jean G Rosario, Stewart A Anderson, Alexandra V Ulyanova, Steven Brem, H Isaac Chen, John A Wolf, M Sean Grady, Sergei A Vinogradov, Junhyong Kim, James Eberwine |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 90 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |