Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene
Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara · 2026
Read Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene by Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/plos-pone-0337675.txt.
Description
Ana Quiroz-Huanca and colleagues sped up a DNA test for a gene that helps E. coli resist a vital family of antibiotics. Their method pairs a faster version of standard DNA copying with a CRISPR-based step that glows when the resistance gene is found. Speeding the heating and cooling cycles cut the copying stage from eighty minutes to about half an hour.
The full check finished in under an hour and still detected mere handfuls of bacteria in spiked poultry droppings, a realistic farm sample. A version using a locally made enzyme took slightly longer but also worked. Because the workflow needs only basic lab equipment and modest skill, the authors pitch it for smaller laboratories that now wait days for resistance answers.
Catalog record
| CREATOR | Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 33508 |
| LICENSE | CC_BY |
| RIGHTS | CC BY |
| SOURCE | PLOS |